我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pCGL0609
- 载体抗性:
- Kanamycin
- 载体长度:
- 6398 bp
- 载体类型:
- Shuttle vector
- 复制子:
- ColA ori
- 载体来源:
- Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
pCGL0609 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pCGL0609 载体序列
LOCUS 40924_10581 6398 bp DNA circular SYN 17-DEC-2018
DEFINITION Shuttle vector pCGL0609, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6398)
AUTHORS Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G.
TITLE 'Integron'-bearing vectors: a method suitable for stable chromosomal
integration in highly restrictive corynebacteria
JOURNAL Gene 107 (1), 61-68 (1991)
PUBMED 1660430
REFERENCE 2 (bases 1 to 6398)
AUTHORS Ankri S, Reyes O, Leblon G.
TITLE Electrotransformation of highly DNA-restrictive corynebacteria with
synthetic DNA
JOURNAL Plasmid 35 (1), 62-66 (1996)
PUBMED 8693028
REFERENCE 3 (bases 1 to 6398)
AUTHORS Ben-Samoun K, Leblon G, Reyes O.
TITLE Positively regulated expression of the Escherichia coli araBAD
promoter in Corynebacterium glutamicum
JOURNAL FEMS Microbiol. Lett. 174 (1), 125-130 (1999)
PUBMED 10234830
REFERENCE 4 (bases 1 to 6398)
AUTHORS Ben-Samoun K, Leblon G, Reyes O.
TITLE Direct Submission
JOURNAL Submitted (17-SEP-1998) Laboratoire de Biologie Moleculaire des
Corynebacteries, Institut de Genetique et Microbiologie URA 2225,
Universite de Paris XI, Orsay F-91405, France
REFERENCE 5 (bases 1 to 6398)
TITLE Direct Submission
REFERENCE 6 (bases 1 to 6398)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
date: "1991"; volume: "107"; issue: "1"; pages: "61-68"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid";
date: "1996"; volume: "35"; issue: "1"; pages: "62-66"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "FEMS
Microbiol. Lett."; date: "1999"; volume: "174"; issue: "1"; pages:
"125-130"
COMMENT SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
(17-SEP-1998) Laboratoire de Biologie Moleculaire des
Corynebacteries, Institut de Genetique et Microbiologie URA 2225,
Universite de Paris XI, Orsay F-91405, France"
COMMENT SGRef: number: 5; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6398
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 72..88
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 675..1466
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
gene complement(2411..3799)
/gene="repBl1"
/label=repBl1
CDS complement(2495..3700)
/codon_start=1
/gene="repBl1"
/product="replicase"
/label=repBl1
/note="minimally known pBl1 region able to promote
replication"
/protein_id="AAD08692.1"
/translation="MYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRWG
SPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSSM
VKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSMF
SRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASKG
SYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDAD
VRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKDV
AAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTILR
TH"
rep_origin complement(5287..5821)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
rep_origin 5889..6317
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"