载体基本信息 | |||
载体名称 | pCPC82 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7419 bp | 载体类型 | Expression vector |
载体来源 | Chang P, Wang W, Chen J. |
pCPC82 载体载体图谱
pCPC82 载体载体序列
LOCUS Exported 7419 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Expression vector pCPC82, complete sequence. ACCESSION MG883684 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7419) AUTHORS Chang P, Wang W, Chen J. TITLE An efficient vector system for economical and convenient epitope-tagging and promoters switching in Candida albicans JOURNAL Unpublished REFERENCE 2 (bases 1 to 7419) AUTHORS Chang P, Wang W, Chen J. TITLE Direct Submission JOURNAL Submitted (31-JAN-2018) Research Center of Bioenergy & Bioremediation, Southwest University, 2 Tiansheng Road, Beibei District, Chongqiong, Chongqing 400715, China REFERENCE 3 (bases 1 to 7419) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7419 /organism="Expression vector pCPC82" /mol_type="other DNA" /db_xref="taxon:2136783" regulatory 1..779 /regulatory_class="promoter" /note="tet_OP4 promoter; tet promoter" protein_bind 124..394 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" misc_feature 798..1505 /note="similar to yeast-enhanced green fluorescent protein; codons optimized for translation in S. cerevisiae and C. albicans; yeGFP" CDS 798..1505 /codon_start=1 /product="yeast-enhanced green fluorescent protein" /label=yeGFP /note="codons optimized for translation in S. cerevisiae and C. albicans" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELY" rep_origin complement(1600..2188) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2359..3219) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3220..3324) /gene="bla" /label=AmpR promoter regulatory 3395..4129 /regulatory_class="promoter" /note="promoter region of ADH1 gene of Candida albicans; CaADH1p" CDS 4130..5113 /codon_start=1 /product="TetR/Gal4AD fusion protein" /label=TetR/Gal4AD fusion protein /note="Candida albicans codon-optimized" /protein_id="AVR52662.1" /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGSTLFGGANFNQSGNI ADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKP LSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE" CDS 4769..5113 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="activation domain of the GAL4 transcriptional activator" /label=GAL4 activation domain /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" regulatory 5128..5434 /regulatory_class="terminator" /note="terminator region of ACT1 gene of Candida albicans; ACT1 terminator" CDS 5781..7187 /codon_start=1 /product="Arg4" /label=Arg4 /protein_id="AVR52663.1" /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL TGTKVYTQGLNKLGLITTEELHLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLK QLENLKSILS"
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