载体基本信息 | |||
载体名称 | pREP | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 11214 bp | 载体类型 | Plant Expression Vectors |
pREP 载体载体图谱
pREP 载体载体序列
LOCUS Exported 11214 bp ds-DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11214) TITLE Direct Submission FEATURES Location/Qualifiers source 1..11214 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..26) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 34..50 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(58..88) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 103..124 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 315..992 /note="CaMV 35S promoter (enhanced)" /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" CDS 1060..2085 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" polyA_signal 2125..2299 /note="CaMV poly(A) signal" /note="cauliflower mosaic virus polyadenylation signal" misc_feature 2377..2401 /note="LB T-DNA repeat" /note="left border repeat from nopaline C58 T-DNA" CDS 2826..3620 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin 3707..4295 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 4481..4621 /note="bom" /note="basis of mobility region from pBR322" rep_origin 4965..5159 /note="pVS1 oriV" /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(5225..6292) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /note="pVS1 RepA" /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" CDS complement(6726..7355) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /note="pVS1 StaA" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 8655..8679 /note="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA" primer_bind 8882..8898 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 9324..9668 /note="CaMV 35S promoter" /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 9728..9752 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(10867..10891) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" terminator 10955..11207 /note="NOS terminator" /note="nopaline synthase terminator and poly(A) signal"
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