- 载体名称:
- pET-28a-EGFP-N
- 载体抗性:
- Kanamycin
- 载体长度:
- 5990 bp
- 载体类型:
- E. coli Expression Vectors, Fluorescent Protein Re
- 复制子:
- ori
- 筛选标记:
- N-EGFP
- 启动子:
- T7/lac
pET-28a-EGFP-N 载体载体图谱
pET-28a-EGFP-N 载体载体序列
LOCUS 40924_17816 5990 bp DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5990) TITLE Direct Submission REFERENCE 2 (bases 1 to 5990) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5990 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 12..467 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(563..1375) /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 1497..2085 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(2271..2413) /label=bom /note="basis of mobility region from pBR322" CDS complement(2518..2706) /label=rop /note="Rop protein, which maintains plasmids at low copy number" protein_bind complement(3481..3502) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(3518..4597) /label=lacI /note="lac repressor" promoter complement(4598..4675) /label=lacI promoter promoter 4984..5002 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 5003..5027 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 5042..5064 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 5071..5787 /label=EGFP /note="enhanced GFP" CDS 5834..5851 /label=6xHis /note="6xHis affinity tag" terminator 5918..5965 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase"
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