载体基本信息 | |||
载体名称 | pGGF001 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3889 bp | 载体类型 | Cloning vector |
载体来源 | Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. |
pGGF001 载体载体图谱
pGGF001 载体载体序列
LOCUS Exported 3889 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pGGF001, complete sequence. ACCESSION KF719007 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3889) AUTHORS Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REFERENCE 2 (bases 1 to 3889) AUTHORS Forner J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany REFERENCE 3 (bases 1 to 3889) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3889 /organism="Cloning vector pGGF001" /mol_type="other DNA" /db_xref="taxon:1439687" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta lactamase" /label=bla /protein_id="AHE38536.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene 201..1061 /gene="bla" /label=bla rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 2212..2230 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" misc_feature 2241..2246 /label=BsaI recognition site /note="BsaI recognition site" misc_feature 2252..3454 /label=pMAS:BastaR:tMAS /note="pMAS:BastaR:tMAS" promoter 2254..2634 /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" CDS 2645..3196 /codon_start=1 /gene="pat" /product="phosphinotricine acetyltransferase" /label=pat /protein_id="AHE38537.1" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" gene 2645..3196 /gene="pat" /label=pat terminator 3202..3454 /label=MAS terminator /note="mannopine synthase terminator" misc_feature complement(3460..3465) /label=BsaI recognition site /note="BsaI recognition site" promoter complement(3476..3494) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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