pGGF001 载体 (V005997#)

载体基本信息
载体名称 pGGF001 载体抗性 Ampicillin
载体长度 3889 bp 载体类型 Cloning vector
载体来源 Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J.

pGGF001 载体载体图谱

pGGF0013889 bp60012001800240030003600AmpR promoterblaoriCAP binding sitelac promoterlac operatorSP6 promoterBsaI recognition sitepMAS:BastaR:tMASBsaI recognition siteT7 promoter

pGGF001 载体载体序列

LOCUS       Exported                3889 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGGF001, complete sequence.
ACCESSION   KF719007
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Forner J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies, 
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg D-69221, Germany
REFERENCE   3  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Cloning vector pGGF001"
                     /mol_type="other DNA"
                     /db_xref="taxon:1439687"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AHE38536.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            201..1061
                     /gene="bla"
                     /label=bla
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2241..2246
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     misc_feature    2252..3454
                     /label=pMAS:BastaR:tMAS
                     /note="pMAS:BastaR:tMAS"
     promoter        2254..2634
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2645..3196
                     /codon_start=1
                     /gene="pat"
                     /product="phosphinotricine acetyltransferase"
                     /label=pat
                     /protein_id="AHE38537.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            2645..3196
                     /gene="pat"
                     /label=pat
     terminator      3202..3454
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    complement(3460..3465)
                     /label=BsaI recognition site
                     /note="BsaI recognition site"
     promoter        complement(3476..3494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"

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