载体基本信息 | |||
载体名称 | pGRB2.1 | 载体抗性 | Ampicillin |
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载体长度 | 5299 bp | 载体类型 | Cloning vector |
载体来源 | Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack BP. |
pGRB2.1 载体载体图谱
pGRB2.1 载体载体序列
LOCUS Exported 5299 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pGRB2.1, complete sequence. ACCESSION KF040395 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5299) AUTHORS Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack BP. TITLE Expression Plasmids for Use in Candida glabrata JOURNAL G3 (Bethesda) 3 (10), 1675-1686 (2013) PUBMED 23934995 REFERENCE 2 (bases 1 to 5299) AUTHORS Zordan RE, Cormack BP. TITLE Direct Submission JOURNAL Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, USA REFERENCE 3 (bases 1 to 5299) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5299 /organism="Cloning vector pGRB2.1" /mol_type="other DNA" /db_xref="taxon:1386884" misc_feature complement(66..664) /label=Candida glabrata CEN/ARS /note="Candida glabrata CEN/ARS" promoter 666..737 /gene="bla" /label=AmpR promoter CDS 738..1598 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" CDS 936..1598 /codon_start=1 /gene="bla" /product="ampicillin-resistance protein" /label=bla /protein_id="AGT40315.1" /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW" gene 936..1598 /gene="bla" /label=bla rep_origin 1693..2375 /label=pBM1(ColE1) origin /note="pBM1(ColE1) origin" rep_origin 1769..2357 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2645..2666 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2681..2711 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2719..2735 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2743..2759 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2780..2798 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 2835..2851 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2885..2901) /label=KS primer /note="common sequencing primer, one of multiple similar variants" regulatory 2904..3301 /regulatory_class="terminator" /note="region downstream of HIS3 orf in Candida glabrata BG2" promoter complement(3316..3334) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3344..3360) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3501..3956 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(4087..4890) /codon_start=1 /gene="URA3" /product="orotidine 5-phosphate decarboxylase" /label=URA3 /protein_id="AGT40316.1" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" gene complement(4087..4890) /gene="URA3" /label=URA3 promoter complement(4891..5106) /gene="S. cerevisiae URA3" /label=URA3 promoter
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