载体基本信息 | |||
载体名称 | pFH2191 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3029 bp | 载体类型 | Cloning vector |
载体来源 | Ostergaard H, Henriksen A, Hansen FG, Winther JR. |
pFH2191 载体载体图谱
pFH2191 载体载体序列
LOCUS Exported 3029 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pFH2191, complete sequence. ACCESSION AF325903 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3029) AUTHORS Ostergaard H, Henriksen A, Hansen FG, Winther JR. TITLE Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein JOURNAL EMBO J. 20 (21), 5853-5862 (2001) PUBMED 11689426 REFERENCE 2 (bases 1 to 3029) AUTHORS Hansen FG. TITLE Synthetic Escherichia coli codon-optimized green fluorescent protein JOURNAL Unpublished REFERENCE 3 (bases 1 to 3029) AUTHORS Hansen FG. TITLE Direct Submission JOURNAL Submitted (04-DEC-2000) Department of Microbiology, Technical University of Denmark, Anker Engelunds Vej, Kgs. Lyngby DK-2800, Denmark REFERENCE 4 (bases 1 to 3029) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3029 /organism="Cloning vector pFH2191" /mol_type="genomic DNA" /db_xref="taxon:153040" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 217..999 /codon_start=1 /product="lacZ-GFP fusion protein" /label=lacZ-GFP fusion protein /note="green fluorescent protein gene codons optimized for Escherichia coli" /protein_id="AAK13447.1" /translation="MTMITPSLHACRSTLEDPRVPHMSKGEELFTGVVPILVELDGDVN GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKRHD FFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKL EYNYNSHNVYIVADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMHELYK" misc_feature 217..282 /label=Region: lacZ /note="Region: lacZ" promoter 1124..1228 /gene="bla" /label=AmpR promoter CDS 1229..2089 /codon_start=1 /product="beta-lactamase" /function="confers resistance to ampicillin" /label=beta-lactamase /protein_id="AAK13448.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2260..2848 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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