pPLc28LIG8 载体 (V004034#)

载体基本信息
载体名称 pPLc28LIG8 载体抗性 Ampicillin
载体长度 4708 bp 载体类型 Bacterial expression vector
载体来源 De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pPLc28LIG8 载体载体图谱

pPLc28LIG84708 bp6001200180024003000360042005' UTR of phage T4 ligase, incl. RBSunnamed protein product; phage T4 ligasebompMB1 oriunnamed protein product; ampicillin resistancePL promoter

pPLc28LIG8 载体载体序列

LOCUS       Exported                4708 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLc28LIG8, complete sequence.
ACCESSION   LT726946
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Bacterial expression vector pPLc28LIG8"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 574."
                     /db_xref="taxon:1944698"
     misc_feature    18..537
                     /label=5' UTR of phage T4 ligase, incl. RBS
                     /note="5' UTR of phage T4 ligase, incl. RBS"
     CDS             538..2001
                     /codon_start=1
                     /note="unnamed protein product; phage T4 ligase"
                     /protein_id="SJL87034.1"
                     /translation="MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQY
                     YIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEV
                     LRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGA
                     RCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHE
                     QVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQV
                     WDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYID
                     QGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECG
                     KIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFL
                     PIAIRLREDKTKANTFEDVFGDFHEVTGL"
     misc_feature    2151..2291
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2293..2695)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2477..3065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3236..4096)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87035.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      4462..4592
                     /regulatory_class="promoter"
                     /note="PL promoter"

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