载体基本信息 | |||
载体名称 | pPpARG4 | 载体抗性 | Ampicillin |
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载体长度 | 5980 bp | 载体类型 | E.coli-K.pastoris shuttle vector |
载体来源 | Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A. |
pPpARG4 载体载体图谱
pPpARG4 载体载体序列
LOCUS Exported 5980 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION E.coli-K.pastoris shuttle vector pPpARG4, complete sequence. ACCESSION JQ519696 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5980) AUTHORS Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A. TITLE Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology JOURNAL PLoS ONE 7 (6), E39720 (2012) PUBMED 22768112 REFERENCE 2 (bases 1 to 5980) AUTHORS Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A. TITLE Direct Submission JOURNAL Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria REFERENCE 3 (bases 1 to 5980) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Assembly Method :: SeqMan v. 7.0.0 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5980 /organism="Escherichia coli-Pichia pastoris shuttle vector pPpARG4" /mol_type="other DNA" /db_xref="taxon:1182032" regulatory 139..1068 /regulatory_class="promoter" /note="AOX1 promoter" regulatory 1097..1356 /regulatory_class="terminator" /note="AOX1 terminator" terminator 1097..1343 /gene="Pichia pastoris AOX1" /label=AOX1 terminator /note="transcription terminator for AOX1" regulatory 1369..2268 /regulatory_class="promoter" /note="ARG4 promoter; derived from Pichia pastoris" CDS 2269..3666 /codon_start=1 /product="argininosuccinate lyase" /label=argininosuccinate lyase /note="ARG4; codon optimized" /protein_id="AFJ20660.1" /translation="MSNQEERLKLWGGRFTGATDPLMDLYNASLPYDKKMYKVDLEGTK VYTEGLEKINLLTKDELSEIHRGLKLIEAEWAEGKFVEKPGDEDIHTANERRLGELIGR GISGKVHTGRSRNDQVATDMRLYVRDNLTQLADYLKQFIQVIIKRAEQEIDVLMPGYTH LQRAQPIRWSHWLSMYATYFTEDYERLNQIVKRLNKSPLGAGALAGHPYGIDREYIAER LGFDSVIGNSLAAVSDRDFVVETMFWSSLFMNHISRFSEDLIIYSTGEFGFIKLADAYS TGSSLMPQKKNPDSLELLRGKSGRCFGALAGFLMSIKSIPSTYNKDMQEDKEPLFDTLI TVEHSILIASGVVSTLNIDAERMKNALTMDMLATDLADYLVRRGVPFRETHHISGECVR QAEELNLSGIDQLSLEQLKSIDSRFEADVASTFDFEASVEKRTATGGTSKTAVLKQLDA LNEKLES" regulatory 3668..4031 /regulatory_class="terminator" /note="ARG4 terminator; derived from Pichia pastoris" regulatory 4032..4089 /regulatory_class="promoter" /note="EM72 synthetic promoter" CDS 4096..4956 /codon_start=1 /product="beta-lactamase" /label=beta-lactamase /note="confers resistance to ampicillin" /protein_id="AFJ20661.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" regulatory 4963..5262 /regulatory_class="terminator" /note="ADH1 terminator" terminator 4963..5150 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" rep_origin 5288..5960 /label=pUC origin of replication /note="pUC origin of replication" rep_origin 5314..5902 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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