我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pRL838
- 载体抗性:
- Chloramphenicol
- 载体长度:
- 8850 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori2
- 载体来源:
- Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M,
pRL838 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pRL838 载体序列
LOCUS V003709 8850 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V003709
VERSION V003709
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8850)
AUTHORS Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A,
Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M,
Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M,
Takazawa M, Yamada M, Yasuda M, Tabata S.
TITLE Complete genomic sequence of the filamentous nitrogen-fixing
cyanobacterium Anabaena sp. strain PCC 7120
JOURNAL DNA Res. 8 (5), 205-213 (2001)
PUBMED 11759840
REFERENCE 2 (bases 1 to 8850)
AUTHORS Wolk CP.
TITLE Direct Submission
JOURNAL Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan
State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE 3 (bases 1 to 8850)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8850)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res.";
date: "2001"; volume: "8"; issue: "5"; pages: "205-213"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan State
University, Wilson Road, E. Lansing, MI 48824-1312, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8850
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 817..1036
/label="ori2"
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
CDS 1127..1879
/label="repE"
/note="replication initiation protein for the bacterial F
plasmid"
misc_feature 1885..2135
/label="incC"
/note="incompatibility region of the bacterial F plasmid"
CDS 2461..3633
/label="sopA"
/note="partitioning protein for the bacterial F plasmid"
CDS 3636..4604
/label="sopB"
/note="partitioning protein for the bacterial F plasmid"
misc_feature 4680..5153
/label="sopC"
/note="centromere-like partitioning region of the bacterial
F plasmid"
CDS complement(5530..5898)
/label="traJ"
/note="oriT-recognizing protein"
oriT complement(5931..6040)
/direction=LEFT
/label="oriT"
/note="incP origin of transfer"
misc_feature 6192..6591
/label="cos"
/note="lambda cos site; allows packaging into phage lambda
particles"
protein_bind complement(6602..6635)
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 6651..6669
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 6673..6832
/label="polylinker (multiple cloning site)"
/note="polylinker (multiple cloning site)"
regulatory 6832..6849
/note="SP6 promoter from pBAC108L (GenBank Accession Number
U51114)"
/regulatory_class="promoter"
promoter complement(6832..6849)
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
regulatory 6865..6891
/note="cat promoter from pBR325 (GenBank Accession Number
L08855)"
/regulatory_class="promoter"
CDS 6958..7614
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 7871..8602
/gene="ermC"
/label="rRNA adenine N-6-methyltransferase"
/note="rRNA adenine N-6-methyltransferase from
Staphylococcus aureus. Accession#: P02979"
terminator 8795..8838
/label="bacterial terminator"
/note="putative bacterial transcription terminator"
regulatory 8801..8829
/note="rrnC terminator from Lorist6 (GenBank Accession
Number X98450)"
/regulatory_class="terminator"