pSolycp00007 载体 (V003118#)

载体基本信息
载体名称 pSolycp00007 载体抗性 Kanamycin
载体长度 11812 bp 载体类型 Cloning vector
载体来源 Aoki K, Kurabayashi A, Suda K, Odahara M, Goto DB, Shibata D.

pSolycp00007 载体载体图谱

pSolycp0000711812 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000105001100011500Right border repeat from C58 T-DNAM13 fwdattB1tomato promoter, BLAST search(vs SL2.40.fasta) result/SL2.40ch02:44011063..44012562attB2fusion protein of histone 2A and enhanced yellonos terminatorM13 revlac operatorlac promoterCAP binding site35S Promoter from CaMV (stop short of BstXI site)nptIICaMV35S polyALeft border repeat from C58 T-DNAKanamycin kinase, type IIIoripBR322 bompVS1 oriVpVS1 RepApVS1 sta

pSolycp00007 载体载体序列

LOCUS       Exported               11812 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSolycp00007 DNA, complete sequence.
ACCESSION   AB684632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto DB, Shibata D.
  TITLE     5'-flanking region of Solyc02g086820 fused to SlHTA6-EYFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto DB, Shibata D.
  TITLE     Tomato HTA6 fused to EYFP driven by 35S omega promoter
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11812)
  AUTHORS   Aoki K, Kurabayashi A, Suda K, Odahara M, Goto D, Shibata D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2011) Contact:Koh Aoki Osaka Prefecture 
            University, Department of Life and Environmental Sciences; Naka-ku, 
            Gakuen-cho, 1-1, Sakai, Osaka 599-8531, Japan E-mail 
            :kaoki@plant.osakafu-u.ac.jp Phone :81-72-254-9421 Fax 
            :81-72-254-9918
REFERENCE   4  (bases 1 to 11812)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Cloning vector pSolycp00007"
                     /mol_type="other DNA"
                     /note="transformation vector for Solanum lycopersicum cv. 
                     Micro-Tom"
                     /db_xref="taxon:1128045"
     misc_feature    complement(1..26)
                     /label=Right border repeat from C58 T-DNA
                     /note="Right border repeat from C58 T-DNA"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    345..369
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(346..369)
                     /label=attB1
                     /note="attB1"
     regulatory      390..1889
                     /regulatory_class="promoter"
                     /note="tomato promoter, BLAST search(vs SL2.40.fasta) 
                     result/SL2.40ch02:44011063..44012562"
     protein_bind    complement(1906..1930)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(1906..1929)
                     /label=attB2
                     /note="attB2"
     CDS             1955..3118
                     /codon_start=1
                     /product="fusion protein of histone 2A and enhanced yellow 
                     fluorescence protein"
                     /label=fusion protein of histone 2A and enhanced yello
                     /protein_id="BAL49731.1"
                     /translation="MDATKTTKGAGGRKGGPRKKSVTKSIKAGLQFPVGRIGRYLKKGR
                     YAQRVGSGAPIYLAAVLEYLAAEVLELAGNAARDNKKSRIIPRHVLLAVRNDEELGKLL
                     AGVTIASGGVLPNINPVLLPKKSAVAEEKSPKAKAGKSPKKALEMVSKGEELFTGVVPI
                     LVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFAR
                     YPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
                     DGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1955..2392
                     /gene="SlHTA6"
                     /note="The nucleotide sequence is identical to GenBank 
                     JN790634.1.; putative histone 2A protein"
     gene            1955..2392
                     /gene="SlHTA6"
                     /label=SlHTA6
     misc_feature    2399..3118
                     /gene="eyfp"
                     /label=enhanced yellow fluorescence protein
                     /note="enhanced yellow fluorescence protein"
     gene            2399..3118
                     /gene="eyfp"
                     /label=eyfp
     CDS             2399..3118
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      complement(3125..3382)
                     /regulatory_class="terminator"
                     /note="nos terminator"
     primer_bind     complement(3404..3420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3428..3444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3452..3482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3497..3518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      3649..4418
                     /regulatory_class="promoter"
                     /note="35S Promoter from CaMV (stop short of BstXI site)"
     promoter        3709..4386
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4449..5246
                     /codon_start=1
                     /gene="nptII"
                     /product="Kanamycin kinase, type II"
                     /label=nptII
                     /note="Kanamycin resistance gene"
                     /protein_id="BAL49732.1"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            4449..5246
                     /gene="nptII"
                     /label=nptII
     regulatory      complement(5289..5504)
                     /regulatory_class="terminator"
                     /note="CaMV35S polyA"
     polyA_signal    5303..5477
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5554..5579)
                     /label=Left border repeat from C58 T-DNA
                     /note="Left border repeat from C58 T-DNA"
     misc_feature    5555..5579
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6004..6798
                     /codon_start=1
                     /product="Kanamycin kinase, type III"
                     /label=Kanamycin kinase, type III
                     /note="kanamycin resistance gene amplified from pIG121Hm"
                     /protein_id="BAL49733.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6885..7473
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      7089..7369
                     /label=pBR322 ori
                     /note="pBR322 ori"
     misc_feature    7509..7769
                     /label=pBR322 bom
                     /note="pBR322 bom"
     misc_feature    7659..7799
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8143..8337
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8179..9179
                     /label=pVS1 rep
                     /note="pVS1 rep"
     CDS             complement(8403..9470)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    9772..10772
                     /label=pVS1 sta
                     /note="pVS1 sta"
     CDS             complement(9904..10533)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

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