pSV23SMTNF 载体 (V002981#)

载体基本信息
载体名称 pSV23SMTNF 载体抗性 Ampicillin
载体长度 5117 bp 载体类型 Mammalian expression vector
载体来源 De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pSV23SMTNF 载体载体图谱

pSV23SMTNF5117 bp6001200180024003000360042004800unnamed protein product; part of SV40 VP1SV40 polyA early3' UTR of SV40 small t-antigenintron of SV40 small t-antigenunnamed protein product; part of SV40 small t-antigen3' UTR of mTNFunnamed protein product; mTNF mature sequenceunnamed protein product; mTNF presequence5' UTR of mTNFSV40 promoterpMB1 oriunnamed protein product; ampicillin resistanceAmpR promoter

pSV23SMTNF 载体载体序列

LOCUS       Exported                5117 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV23SMTNF, complete sequence.
ACCESSION   LT727628
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5117)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Mammalian expression vector pSV23SMTNF"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2171."
                     /db_xref="taxon:1945078"
     CDS             1..809
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 VP1"
                     /protein_id="SJL89019.1"
                     /translation="FLCLI*MRT*PVEIF*CGKLLLLKLRLLG*LLC*TCIQGHKKLMK
                     MVLENPFKGQIFIFLLLVGNLWSCRVC*QTTGPNILLKL*PQKMLQLTVSR*TLTTRLF
                     WIRIMLIQWSAGFLIQVKMKTLDILEPTQVGKMCLLFCTLLTQQPQCFLMSRVLGPCAK
                     LTACMFLLLTFVGCLPTLLEHSSGRDFPDILKLPLESGL*KTPTQFPFC*VT*LTGGHR
                     GWMGSL*LECPLK*RRLGFMRTQRSFLGIQT**DTLMSLDKPQLECS"
     polyA_signal    750..884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(810..906)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      810..906
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(907..1458)
                     /label=3' UTR of SV40 small t-antigen
                     /note="3' UTR of SV40 small t-antigen"
     CDS             complement(1309..1329)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1459..1525)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1459..1524
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL89020.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1624..1781)
                     /label=3' UTR of mTNF
                     /note="3' UTR of mTNF"
     CDS             complement(1782..2252)
                     /codon_start=1
                     /note="unnamed protein product; mTNF mature sequence"
                     /protein_id="SJL89021.1"
                     /translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKD
                     NQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDT
                     PEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
     CDS             complement(2253..2489)
                     /codon_start=1
                     /note="unnamed protein product; mTNF presequence"
                     /protein_id="SJL89022.1"
                     /translation="MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGAT
                     TLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLT"
     misc_feature    complement(2490..2517)
                     /label=5' UTR of mTNF
                     /note="5' UTR of mTNF"
     promoter        complement(2546..2875)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2560..2695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2571..2646)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     regulatory      complement(2603..2884)
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      complement(3106..3508)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3290..3878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4049..4909)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL89023.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4910..5014)
                     /gene="bla"
                     /label=AmpR promoter

我们不提供该载体,载体信息仅供查询和参考。