载体基本信息 | |||
载体名称 | pSV23SMTNF | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5117 bp | 载体类型 | Mammalian expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pSV23SMTNF 载体载体图谱
pSV23SMTNF 载体载体序列
LOCUS Exported 5117 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Mammalian expression vector pSV23SMTNF, complete sequence. ACCESSION LT727628 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5117) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5117) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5117) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5117 /organism="Mammalian expression vector pSV23SMTNF" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2171." /db_xref="taxon:1945078" CDS 1..809 /codon_start=1 /note="unnamed protein product; part of SV40 VP1" /protein_id="SJL89019.1" /translation="FLCLI*MRT*PVEIF*CGKLLLLKLRLLG*LLC*TCIQGHKKLMK MVLENPFKGQIFIFLLLVGNLWSCRVC*QTTGPNILLKL*PQKMLQLTVSR*TLTTRLF WIRIMLIQWSAGFLIQVKMKTLDILEPTQVGKMCLLFCTLLTQQPQCFLMSRVLGPCAK LTACMFLLLTFVGCLPTLLEHSSGRDFPDILKLPLESGL*KTPTQFPFC*VT*LTGGHR GWMGSL*LECPLK*RRLGFMRTQRSFLGIQT**DTLMSLDKPQLECS" polyA_signal 750..884 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory complement(810..906) /regulatory_class="terminator" /note="SV40 polyA early" regulatory 810..906 /regulatory_class="terminator" /note="SV40 polyA late" misc_feature complement(907..1458) /label=3' UTR of SV40 small t-antigen /note="3' UTR of SV40 small t-antigen" CDS complement(1309..1329) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature complement(1459..1525) /label=intron of SV40 small t-antigen /note="intron of SV40 small t-antigen" intron 1459..1524 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS complement(1526..1600) /codon_start=1 /note="unnamed protein product; part of SV40 small t-antigen" /protein_id="SJL89020.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" misc_feature complement(1624..1781) /label=3' UTR of mTNF /note="3' UTR of mTNF" CDS complement(1782..2252) /codon_start=1 /note="unnamed protein product; mTNF mature sequence" /protein_id="SJL89021.1" /translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKD NQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDT PEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL" CDS complement(2253..2489) /codon_start=1 /note="unnamed protein product; mTNF presequence" /protein_id="SJL89022.1" /translation="MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGAT TLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLT" misc_feature complement(2490..2517) /label=5' UTR of mTNF /note="5' UTR of mTNF" promoter complement(2546..2875) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2560..2695 /label=SV40 ori /note="SV40 origin of replication" rep_origin complement(2571..2646) /direction=LEFT /label=SV40 ori /note="SV40 ori" regulatory complement(2603..2884) /regulatory_class="promoter" /note="SV40 early promoter" rep_origin complement(3106..3508) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(3290..3878) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4049..4909) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL89023.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4910..5014) /gene="bla" /label=AmpR promoter
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