- 载体名称:
- pSV23SMTNF
- 载体抗性:
- Ampicillin
- 载体长度:
- 5117 bp
- 载体类型:
- Mammalian expression vector
- 复制子:
- ori
- 载体来源:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- 启动子:
- SV40
pSV23SMTNF 载体载体图谱
pSV23SMTNF 载体载体序列
LOCUS 40924_41629 5117 bp DNA circular SYN 18-DEC-2018 DEFINITION Mammalian expression vector pSV23SMTNF, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5117) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5117) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5117) TITLE Direct Submission REFERENCE 4 (bases 1 to 5117) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5117 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(750..884) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(1309..1329) /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" intron complement(1459..1524) /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS complement(1526..1600) /codon_start=1 /note="unnamed protein product; part of SV40 small t-antigen" /protein_id="SJL89020.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" misc_feature complement(1624..1781) /label=3' UTR of mTNF /note="3' UTR of mTNF" CDS complement(1785..2489) /gene="Tnf" /label=Tnf /note="Tumor necrosis factor from Mus musculus. Accession#: P06804" misc_feature complement(2490..2517) /label=5' UTR of mTNF /note="5' UTR of mTNF" promoter complement(2546..2875) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin complement(3290..3878) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4052..4909) /label=AmpR /note="beta-lactamase" promoter complement(4910..5014) /label=AmpR promoter
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