pSV55 载体 (V002942#)

载体基本信息
载体名称 pSV55 载体抗性 Ampicillin
载体长度 8420 bp 载体类型 Mammalian expression vector
载体来源 De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pSV55 载体载体图谱

pSV558420 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400SV40 early promoterlarge T antigenintronSV40 large T-antigenpolyAintron5UTRSV40 late promoterunnamed protein product; SV40 small t-antigenpart of SV40 large t-antigenbompMB1 oriunnamed protein product; ampicillin resistanceAmpR promoter

pSV55 载体载体序列

LOCUS       Exported                8420 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV55, complete sequence.
ACCESSION   LT727594
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Mammalian expression vector pSV55"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1833."
                     /db_xref="taxon:1945117"
     regulatory      1..278
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     promoter        6..335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      186..321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      235..310
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             join(349..594,941..2821)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             349..873
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88937.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    349..594
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    595..940
                     /label=intron
                     /note="intron"
     intron          595..940
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    941..2821
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     regulatory      complement(2822..2918)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2822..2918
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    2844..2978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label=intron
                     /note="intron"
     misc_feature    complement(3953..4155)
                     /label=5UTR
                     /note="5UTR"
     regulatory      complement(4188..4455)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        4192..4549
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4211..4492
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4400..4535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4449..4524
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4563..5087
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88938.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    4563..4808
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    4809..5154
                     /label=intron
                     /note="intron"
     intron          4809..5154
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    5155..6217
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             5284..5304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6359..6499
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6500..6900)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(6685..7273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7444..8304)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88939.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8305..8409)
                     /gene="bla"
                     /label=AmpR promoter

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