pSV55 载体 (V002942#)

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。

所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。

由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。

载体名称:
pSV55
载体抗性:
Ampicillin
载体长度:
8420 bp
载体类型:
Mammalian expression vector
复制子:
ori
载体来源:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pSV55 载体载体图谱

pSV558420 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400SV40 promotersmall t antigensmall t intronSV40 NLSSV40 poly(A) signalintronAgnoproteinSV40 promotersmall t antigenSV40 NLSbomoriAmpRAmpR promoter

pSV55 载体载体序列

LOCUS       V002942                 8420 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002942
VERSION     V002942
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..316
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             349..870
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          875..940
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1070..1090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2844..2978
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label="intron"
                     /note="intron"
     CDS             complement(3959..4144)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4192..4549
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4563..5084
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             5284..5304
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    6359..6499
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6685..7273)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7447..8304)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8305..8409)
                     /label="AmpR promoter"

我们不提供该载体,载体信息仅供查询和参考。