下载GeneBank文件(.gb)

LOCUS       Exported                8420 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSV55, complete sequence.
ACCESSION   LT727594
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Mammalian expression vector pSV55"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1833."
                     /db_xref="taxon:1945117"
     regulatory      1..278
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     promoter        6..335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      186..321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      235..310
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             join(349..594,941..2821)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     CDS             349..873
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88937.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    349..594
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    595..940
                     /label=intron
                     /note="intron"
     intron          595..940
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    941..2821
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     regulatory      complement(2822..2918)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2822..2918
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_signal    2844..2978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3015..3952)
                     /label=intron
                     /note="intron"
     misc_feature    complement(3953..4155)
                     /label=5UTR
                     /note="5UTR"
     regulatory      complement(4188..4455)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        4192..4549
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4211..4492
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4400..4535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4449..4524
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4563..5087
                     /codon_start=1
                     /note="unnamed protein product; SV40 small t-antigen"
                     /protein_id="SJL88938.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
     misc_feature    4563..4808
                     /label=SV40 large T-antigen
                     /note="SV40 large T-antigen"
     misc_feature    4809..5154
                     /label=intron
                     /note="intron"
     intron          4809..5154
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     misc_feature    5155..6217
                     /label=part of SV40 large t-antigen
                     /note="part of SV40 large t-antigen"
     CDS             5284..5304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6359..6499
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6500..6900)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(6685..7273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7444..8304)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88939.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8305..8409)
                     /gene="bla"
                     /label=AmpR promoter