载体基本信息 | |||
载体名称 | pSV55 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 8420 bp | 载体类型 | Mammalian expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pSV55 载体载体图谱
pSV55 载体载体序列
LOCUS Exported 8420 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Mammalian expression vector pSV55, complete sequence. ACCESSION LT727594 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8420) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 8420) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 8420) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..8420 /organism="Mammalian expression vector pSV55" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 1833." /db_xref="taxon:1945117" regulatory 1..278 /regulatory_class="promoter" /note="SV40 early promoter" promoter 6..335 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 186..321 /label=SV40 ori /note="SV40 origin of replication" rep_origin 235..310 /label=SV40 ori /note="SV40 ori" CDS join(349..594,941..2821) /codon_start=1 /product="SV40 (simian virus 40) large T antigen" /label=large T antigen /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT CFKKPPTPPPEPET" CDS 349..873 /codon_start=1 /note="unnamed protein product; SV40 small t-antigen" /protein_id="SJL88937.1" /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI IGQTTYRDLKL" misc_feature 349..594 /label=SV40 large T-antigen /note="SV40 large T-antigen" misc_feature 595..940 /label=intron /note="intron" intron 595..940 /label=large T intron /note="SV40 (simian virus 40) large T antigen intron" misc_feature 941..2821 /label=SV40 large T-antigen /note="SV40 large T-antigen" regulatory complement(2822..2918) /regulatory_class="terminator" /note="polyA" regulatory 2822..2918 /regulatory_class="terminator" /note="polyA" polyA_signal 2844..2978 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature complement(3015..3952) /label=intron /note="intron" misc_feature complement(3953..4155) /label=5UTR /note="5UTR" regulatory complement(4188..4455) /regulatory_class="promoter" /note="SV40 late promoter" promoter 4192..4549 /label=SV40 promoter /note="SV40 enhancer and early promoter" regulatory 4211..4492 /regulatory_class="promoter" /note="SV40 early promoter" rep_origin 4400..4535 /label=SV40 ori /note="SV40 origin of replication" rep_origin 4449..4524 /label=SV40 ori /note="SV40 ori" CDS 4563..5087 /codon_start=1 /note="unnamed protein product; SV40 small t-antigen" /protein_id="SJL88938.1" /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI IGQTTYRDLKL" misc_feature 4563..4808 /label=SV40 large T-antigen /note="SV40 large T-antigen" misc_feature 4809..5154 /label=intron /note="intron" intron 4809..5154 /label=large T intron /note="SV40 (simian virus 40) large T antigen intron" misc_feature 5155..6217 /label=part of SV40 large t-antigen /note="part of SV40 large t-antigen" CDS 5284..5304 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 6359..6499 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(6500..6900) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(6685..7273) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7444..8304) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL88939.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8305..8409) /gene="bla" /label=AmpR promoter
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