- 载体名称:
- pTac-5'UTR-Sgr_rppA
- 载体抗性:
- Streptomycin
- 载体长度:
- 4820 bp
- 载体类型:
- Cloning vector
- 复制子:
- CloDF13 ori
- 载体来源:
- Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
pTac-5'UTR-Sgr_rppA 载体载体图谱
pTac-5'UTR-Sgr_rppA 载体载体序列
LOCUS 40924_42419 4820 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTac-5'UTR-Sgr_rppA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4820) AUTHORS Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY. TITLE Direct Submission JOURNAL Submitted (12-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon 34141, South Korea REFERENCE 2 (bases 1 to 4820) TITLE Direct Submission REFERENCE 3 (bases 1 to 4820) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (12-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon 34141, South Korea" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..4820 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 175..203 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 211..227 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 5'UTR 231..255 /gene="rppA" CDS 256..1371 /gene="rppA" /label=rppA /note="1,3,6,8-tetrahydroxynaphthalene synthase from Streptomyces griseus. Accession#: Q54240" regulatory 1418..1565 /label=rrnBT1T2 /note="rrnBT1T2" /regulatory_class="terminator" CDS complement(1739..2527) /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" promoter complement(2528..2619) /label=AmpR promoter rep_origin complement(2667..3405) /direction=LEFT /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." protein_bind complement(3581..3602) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(3618..4697) /label=lacI /note="lac repressor" promoter complement(4698..4775) /label=lacI promoter
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