载体基本信息 | |||
载体名称 | pTac-5'UTR-Sgr_rppA | 载体长度 | 4820 bp |
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载体类型 | Cloning vector | 载体来源 | Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY. |
pTac-5'UTR-Sgr_rppA 载体载体图谱
pTac-5'UTR-Sgr_rppA 载体载体序列
LOCUS Exported 4820 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTac-5'UTR-Sgr_rppA, complete sequence. ACCESSION MH473345 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4820) AUTHORS Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY. TITLE Direct Submission JOURNAL Submitted (12-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon 34141, South Korea REFERENCE 2 (bases 1 to 4820) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4820 /organism="Cloning vector pTac-5'UTR-Sgr_rppA" /lab_host="Escherichia coli" /mol_type="other DNA" /db_xref="taxon:2294147" regulatory 175..203 /regulatory_class="promoter" /note="Tac promoter" promoter 175..203 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 211..227 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." gene 231..1374 /gene="rppA" /label=rppA 5'UTR 231..255 /gene="rppA" CDS 256..1374 /codon_start=1 /gene="rppA" /product="1,3,6,8-tetrahydroxynaphthalene synthase" /label=rppA /note="1,3,6,8-tetrahydroxynaphthalene synthase from Streptomyces griseus" /protein_id="AXN69936.1" /translation="MATLCRPAIAVPEHVITMQQTLDLARETHAGHPQRDLVLRLIQNT GVQTRHLVQPIEKTLAHPGFEVRNQVYEAEAKTRVPEVVRRALANAETEPSEIDLIVYV SCTGFMMPSLTAWIINSMGFRPETRQLPIAQLGCAAGGAAINRAHDFCVAYPDSNVLIV SCEFCSLCYQPTDIGVGSLLSNGLFGDALSAAVVRGQGGTGMRLERNGSHLVPDTEDWI SYAVRDTGFHFQLDKRVPGTMEMLAPVLLDLVDLHGWSVPNMDFFIVHAGGPRILDDLC HFLDLPPEMFRYSRATLTERGNIASSVVFDALARLFDDGGAAESAQGLIAGFGPGITAE VAVGSWAKEGLGADVGRDLDELELTAGVALSG" regulatory 1418..1565 /regulatory_class="terminator" /note="rrnBT1T2" CDS complement(1736..2527) /codon_start=1 /product="spectinomycin resistance protein" /label=spectinomycin resistance protein /protein_id="AXN69937.1" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" promoter complement(2528..2619) /gene="bla" /label=AmpR promoter rep_origin 2667..3405 /label=CDF /note="CDF" CDS complement(3615..4697) /codon_start=1 /gene="lacIq" /product="lac repressor protein" /label=lacIq /protein_id="AXN69938.1" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" gene complement(3615..4697) /gene="lacIq" /label=lacIq promoter complement(4698..4775) /gene="lacI" /label=lacI promoter
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