pTcR-HG-PAC-plus 载体 (V002765#)

载体基本信息
载体名称 pTcR-HG-PAC-plus 载体抗性 Ampicillin
载体长度 3340 bp 载体类型 Cloning vector
载体来源 Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira CA.

pTcR-HG-PAC-plus 载体载体图谱

pTcR-HG-PAC-plus3340 bp6001200180024003000MCSPACMCSAmpR

pTcR-HG-PAC-plus 载体载体序列

LOCUS       Exported                3340 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-PAC-plus, complete sequence.
ACCESSION   JN596077
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3340)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Cloning vector pTcR-HG-PAC-plus"
                     /mol_type="other DNA"
                     /db_xref="taxon:1133384"
     misc_feature    1..52
                     /label=MCS
                     /note="MCS"
     intron          53..263
                     /gene="HX1"
     gene            53..263
                     /gene="HX1"
                     /label=HX1
     CDS             266..865
                     /codon_start=1
                     /gene="PAC"
                     /product="PAC"
                     /label=PAC
                     /note="puromycin N-acetyl-transferase"
                     /protein_id="AEZ51441.1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVEVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPEHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     gene            266..865
                     /gene="PAC"
                     /label=PAC
     intron          866..1381
                     /gene="IgapdhII"
     gene            866..1381
                     /gene="IgapdhII"
                     /label=IgapdhII
     misc_feature    1381..1436
                     /label=MCS
                     /note="MCS"
     rep_origin      complement(1560..2233)
                     /direction=LEFT
     rep_origin      complement(1619..2207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2378..3238)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /note="beta-lactamase"
                     /protein_id="AEZ51442.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2378..3238)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(3239..3)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3239..3337)
                     /regulatory_class="promoter"
                     /gene="bla"
     gene            complement(3239..3337)
                     /gene="bla"
                     /label=bla

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