载体基本信息 | |||
载体名称 | pTEX5501ts | 载体抗性 | Chloramphenicol |
---|---|---|---|
载体长度 | 6279 bp | 载体类型 | Shuttle vector |
载体来源 | Nallapareddy SR, Singh KV, Murray BE. |
pTEX5501ts 载体载体图谱
pTEX5501ts 载体载体序列
LOCUS Exported 6279 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector pTEX5501ts, complete sequence. ACCESSION DQ208937 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6279) AUTHORS Nallapareddy SR, Singh KV, Murray BE. TITLE Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains JOURNAL Appl. Environ. Microbiol. 72 (1), 334-345 (2006) PUBMED 16391062 REFERENCE 2 (bases 1 to 6279) AUTHORS Nallapareddy SR, Singh KV, Murray BE. TITLE Direct Submission JOURNAL Submitted (18-SEP-2005) Infectious Diseases, Univ. of Texas Med. School at Houston, 6431 Fannin St, MSB 2.112, Houston, TX 77030, USA REFERENCE 3 (bases 1 to 6279) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6279 /organism="Shuttle vector pTEX5501ts" /mol_type="other DNA" /db_xref="taxon:352214" misc_feature 1..61 /note="multiple cloning site I; MCS-I" CDS 195..845 /codon_start=1 /gene="cat" /product="CAT" /label=cat /note="chloramphenicol acetyltransferase; promoter (synthetic aad9) derived from pFW11" /protein_id="ABB20800.1" /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL" gene 195..845 /gene="cat" /label=cat misc_feature 1007..1076 /note="multiple cloning site II; MCS-II" CDS complement(1479..1490) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 1624..1833 /codon_start=1 /gene="orfB" /product="OrfB" /label=orfB /note="portion of pWV01 based temperature-sensitive gram-positive replicon derived from pTV1-OK" /protein_id="ABB20801.1" /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES PTFSPTFFINLYLSSFIVVFMITK" gene 1624..1833 /gene="orfB" /label=orfB CDS 1874..2035 /codon_start=1 /product="OrfC" /label=OrfC /note="portion of pWV01 based temperature-sensitive gram-positive replicon derived from pTV1-OK" /protein_id="ABB20802.1" /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA RKESEQKK" CDS 2102..2800 /codon_start=1 /gene="repA(ts)" /product="RepA(ts)" /label=repA(ts) /note="portion of pWV01 based temperature-sensitive gram-positive replicon derived from pTV1-OK" /protein_id="ABB20803.1" /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDLYITLDESQKRELKNLLLDIV DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK VLDAETGEIK" gene 2102..2800 /gene="repA(ts)" /label=repA(ts) CDS 2797..2946 /codon_start=1 /gene="orfD" /product="OrfD" /label=orfD /note="portion of pWV01 based temperature-sensitive gram-positive replicon derived from pTV1-OK" /protein_id="ABB20804.1" /translation="MTNKEKELFAENEELKKEIKDLKERIERYREMEVELSTTIDLLRG GIIE" gene 2797..2946 /gene="orfD" /label=orfD CDS 3173..4078 /codon_start=1 /gene="aph(2'')-Id" /product="APH(2'')-Id" /label=aph(2'')-Id /note="aminoglycoside modifying enzyme; derived from Enterococcus casseliflavus in GenBank Accession Number AF016483" /protein_id="ABB20805.1" /translation="MRTYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIF KFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLT PLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSR ELKGPQMKIVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAA ISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKE YGYMDWYEEGLNEIRSIKIK" gene 3173..4078 /gene="aph(2'')-Id" /label=aph(2'')-Id oriT 4172..4838 /note="oriT of Tn916 derived from pHS1; facilitates conjugative transfer from the transformable E. faecalis strain JH2Sm::Tn916 to E. faecium" rep_origin 5036..5634 /note="Escherichia coli OriR from plasmid pUC18; derived from pFW14" rep_origin complement(5036..5624) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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