载体基本信息 | |||
载体名称 | pFA6a-TRP1-PGAL1-GFP | 载体抗性 | Ampicillin |
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载体长度 | 4881 bp | 载体类型 | Yeast Plasmids |
载体来源 | Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, | 拷贝数 | High copy number |
pFA6a-TRP1-PGAL1-GFP 载体载体图谱
pFA6a-TRP1-PGAL1-GFP 载体载体序列
LOCUS Exported 4881 bp ds-DNA circular SYN 29-11-2013 DEFINITION Plasmid with a TRP1 marker for swapping in the GAL1 promoter and adding a GFP tag. ACCESSION . VERSION . KEYWORDS pFA6a-TRP1-PGAL1-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4881) AUTHORS Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. TITLE Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. JOURNAL Yeast 1998;14:953-61. PUBMED 9717241 REFERENCE 2 (bases 1 to 4881) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4881 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" terminator 50..237 /note="ADH1 terminator" CDS complement(258..970) /codon_start=3 /gene="gfp" /product="green fluorescent protein, S65T variant" /note="GFP(S65T)" /db_xref="GI:1019894" /protein_id="AAA79306.1" /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV TAAGITHGMDELYK" CDS complement(984..986) /codon_start=1 /note="start codon" /translation="M" promoter complement(1006..1447) /gene="S. cerevisiae GAL1" /note="GAL1 promoter" /note="inducible promoter, regulated by Gal4" protein_bind 1330..1447 /bound_moiety="Gal4" /note="UAS" /note="upstream activating sequence mediating Gal4-dependent induction" promoter 1542..1689 /gene="S. cerevisiae TRP1" /note="TRP1 promoter" CDS 1690..2364 /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /note="TRP1" /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" promoter complement(2519..2537) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2795..3383) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(3554..4414) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4415..4519) /gene="bla" /note="AmpR promoter" promoter 4865..2 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase"
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