| 载体基本信息 | |||
| 载体名称 | pFA6a-TRP1-PGAL1-GFP | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 4881 bp | 载体类型 | Yeast Plasmids |
| 载体来源 | Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, | 拷贝数 | High copy number |
pFA6a-TRP1-PGAL1-GFP 载体载体图谱
pFA6a-TRP1-PGAL1-GFP 载体载体序列
LOCUS Exported 4881 bp ds-DNA circular SYN 29-11-2013
DEFINITION Plasmid with a TRP1 marker for swapping in the GAL1 promoter and
adding a GFP tag.
ACCESSION .
VERSION .
KEYWORDS pFA6a-TRP1-PGAL1-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4881)
AUTHORS Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A,
Philippsen P, Pringle JR.
TITLE Additional modules for versatile and economical PCR-based gene
deletion and modification in Saccharomyces cerevisiae.
JOURNAL Yeast 1998;14:953-61.
PUBMED 9717241
REFERENCE 2 (bases 1 to 4881)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4881
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
terminator 50..237
/note="ADH1 terminator"
CDS complement(258..970)
/codon_start=3
/gene="gfp"
/product="green fluorescent protein, S65T variant"
/note="GFP(S65T)"
/db_xref="GI:1019894"
/protein_id="AAA79306.1"
/translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
TAAGITHGMDELYK"
CDS complement(984..986)
/codon_start=1
/note="start codon"
/translation="M"
promoter complement(1006..1447)
/gene="S. cerevisiae GAL1"
/note="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
protein_bind 1330..1447
/bound_moiety="Gal4"
/note="UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 1542..1689
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
CDS 1690..2364
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/note="TRP1"
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
promoter complement(2519..2537)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2795..3383)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3554..4414)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4415..4519)
/gene="bla"
/note="AmpR promoter"
promoter 4865..2
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
我们不提供该载体，载体信息仅供查询和参考。