载体基本信息 | |||
载体名称 | pLenti6/TR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 8354 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
pLenti6/TR 载体载体图谱
pLenti6/TR 载体载体序列
LOCUS Exported 8354 bp ds-DNA circular SYN 10-1-2013 DEFINITION Lentiviral vector for high-level constitutive expression of the Tet repressor. ACCESSION . VERSION . KEYWORDS pLenti6 TR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8354) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission FEATURES Location/Qualifiers source 1..8354 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" promoter 1..229 /note="RSV promoter" /note="Rous Sarcoma Virus enhancer/promoter" LTR 230..410 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 457..582 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1075..1308 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." enhancer 1816..2119 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 2120..2323 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" intron 2552..3124 /note="beta-globin intron" /note="intron from rabbit beta-globin gene" CDS 3208..3828 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /note="TetR" /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /protein_id=" " /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" promoter 3947..4276 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 4127..4262 /note="SV40 ori" /note="SV40 origin of replication" promoter 4324..4371 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 4390..4788 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /note="BSD" /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" LTR 4875..5108 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5180..5301 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 5683..6138 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6164..6268 /gene="bla" /note="AmpR promoter" CDS 6269..7129 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7300..7888 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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