- 载体名称:
- pCMV-Red Firefly Luc
- 载体抗性:
- Ampicillin
- 载体长度:
- 6575 bp
- 载体类型:
- Luciferase Vectors
- 复制子:
- ori
- 载体来源:
- Thermo Scientific (Pierce)
- 拷贝数:
- High copy number
- 启动子:
- SV40
pCMV-Red Firefly Luc 载体载体图谱
pCMV-Red Firefly Luc 载体载体序列
LOCUS 40924_11740 6575 bp DNA circular SYN 01-JAN-1980 DEFINITION Control vector for constitutive high-level expression of intracellular red firefly luciferase under control of the CMV promoter. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6575) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 6575) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6575 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 64..367 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 368..571 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 616..634 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 647..2290 /codon_start=1 /label=Red Firefly Luciferase /note="intracellular red firefly luciferase" /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG VRFVDEVPKGLTGKIDGRAIREILKKPVAKM" polyA_signal 2319..2430 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 2553..2882 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 2930..2977 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2996..3592 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 3725..3858 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(3902..4759) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5023..5611 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5881..5897 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 6353..6369 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 6484..6575 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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