载体基本信息 | |||
载体名称 | pCMV-Green Renilla Luc | 载体抗性 | Ampicillin |
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载体长度 | 5860 bp | 载体类型 | Luciferase Vectors |
载体来源 | Thermo Scientific (Pierce) | 拷贝数 | High copy number |
pCMV-Green Renilla Luc 载体载体图谱
pCMV-Green Renilla Luc 载体载体序列
LOCUS Exported 5860 bp ds-DNA circular SYN 26-7-2013 DEFINITION Control vector for constitutive high-level expression of intracellular green Renilla luciferase under control of the CMV promoter. ACCESSION . VERSION . KEYWORDS pCMV-Green Renilla Luc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5860) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission FEATURES Location/Qualifiers source 1..5860 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 63..366 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 367..570 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 615..633 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 646..1581 /codon_start=1 /product="green-emitting variant of Renilla luciferase" /note="Green Renilla Luciferase" /note="human codon-optimized" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 1604..1715 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" promoter 1838..2167 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2018..2153 /note="SV40 ori" /note="SV40 origin of replication" promoter 2215..2262 /note="EM7 promoter" /note="synthetic bacterial promoter " CDS 2281..2880 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 3010..3131 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" CDS complement(3184..4044) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4308..4896 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5638..5654 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 5769..5860 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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