载体基本信息 | |||
载体名称 | pE2-Crimson | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3311 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
3'引物 | M13 rev |
pE2-Crimson 载体载体图谱
pE2-Crimson 载体载体序列
LOCUS Exported 3311 bp ds-DNA circular SYN 26-11-2013 DEFINITION Vector for expressing rapidly maturing and noncytotoxic E2-Crimson in bacteria. ACCESSION . VERSION . KEYWORDS pE2-Crimson SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3311) AUTHORS Clontech TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..3311 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 143..173 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 181..197 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 217..219 /codon_start=1 /product="lacZ start codon" /note="ATG" /translation="M" CDS 289..966 /codon_start=1 /product="far-red noncytotoxic tetrameric variant of DsRed fluorescent protein" /note="E2-Crimson" /note="mammalian codon-optimized" /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH LFQ" promoter 1406..1510 /gene="bla" /note="AmpR promoter" CDS 1511..2371 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2542..3130 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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