载体基本信息 | |||
载体名称 | pAM656 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5255 bp | 载体类型 | Saccharomyces cerevisiae vector |
载体来源 | Agaphonov M, Alexandrov A. |
pAM656 载体载体图谱
pAM656 载体载体序列
LOCUS Exported 5255 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Saccharomyces cerevisiae vector pAM656, complete sequence. ACCESSION KJ912895 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5255) AUTHORS Agaphonov M, Alexandrov A. TITLE Self-excising integrative yeast plasmid vectors containing an intronated recombinase gene JOURNAL FEMS Yeast Res. (2014) In press PUBMED 25124534 REFERENCE 2 (bases 1 to 5255) AUTHORS Agaphonov M, Alexandrov A. TITLE Direct Submission JOURNAL Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy of Science, Moscow 119071, Russian Federation REFERENCE 3 (bases 1 to 5255) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5255 /organism="Saccharomyces cerevisiae vector pAM656" /mol_type="other DNA" /db_xref="taxon:1538092" primer_bind complement(22..38) /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_recomb complement(51..84) /label=loxP /note="loxP" protein_bind complement(51..84) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter complement(109..550) /gene="S. cerevisiae GAL1" /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" protein_bind 433..550 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" CDS join(681..683,735..1724) /codon_start=1 /product="Cre" /label=Cre /protein_id="AIL02111.1" /translation="MSIDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKL NNREWFPAEPEDVRGYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVM RRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAE IARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPN NYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVG AARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD" intron 684..734 rep_origin complement(1797..2385) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2556..3416) /codon_start=1 /product="beta-lactamase" /label=beta-lactamase /protein_id="AIL02112.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3417..3521) /gene="bla" /label=AmpR promoter promoter 3627..4029 /gene="S. cerevisiae LEU2" /label=LEU2 promoter CDS 4030..5124 /codon_start=1 /product="beta-isopropylmalate dehydrogenase" /label=beta-isopropylmalate dehydrogenase /protein_id="AIL02113.1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" misc_recomb complement(5195..5228) /label=loxP /note="loxP" protein_bind complement(5195..5228) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
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